Some of the thoughts that come to mind are:
1) Do you have a national CSSD standard that you follow in Pakistan?
If yes, What does the standard say?
2) What does your Manufacturer say?
We have to take into consideration whatever is recommended by the Manufacturer of the said steriliser.
Depending on which part of the world influences you, We will have different practices.
The Americans are big on biological indicators. Some do it everyday, may be also once every shift.
The Europeans are not necessarily fans of the this concept. They rely more on Parametric release.
I work in New Zealand. We follow AS/NZS 4187:2014 which is greatly influenced by various European and ISO standards.
For Steam Sterilisation:
Biological incubation is optional for regular monitoring, though recommended as best practice. In NZ, it is mandatory to include biological indicators while processing implants through steam sterilisation. If used, the indicator vial shall be placed just above the drain. The drain is the cold spot and hence needs to be monitored/challenged.
For Hydrogen Peroxide (Plasma / VHP):
In NZ, it is mandatory to use biological monitoring at least once a week. Depending on the manufacturer’s instructions, these indicator vials are placed in the recommended locations.
For ETO sterilisation:
In NZ, we have to use BIs in every load of ETO sterilisation. Also, depending on the validation studies, the manufacturer will recommend whether one vial is enough or whether multiple vials should be used in multiple locations.
Up until a few years ago, the process of biological incubation was very time consuming. It would take 24 hours for the results to be seen. This made it impractical for regular use. Hence, the process of parametric release was so popular.
However, this is no longer the case. Biological incubation results can be recorded in 24 minutes.
The process of sterilisation can be monitored by 3 methods, viz, Physical, Chemical & Biological. When used in combination, all the 3 methods complement each other.
Dear Shoeb, I hope your questions have been answered.
Always do your best to follow your local standards/guidelines.
I take this opportunity to wish you the very best in your career in Sterile Sciences.